| Route | From | To | Dates | Now |
| 321 | Chesahowitska Springs | Inverness | 1937 | |
| 322 | Dade City | Orlando | 1937 | |
| 323 | Tampa | Port Tampa | 1937 | |
| 324 | Bartow | Babson Park | 1937-1945 | SR 60, CR 559, Alturas - Babson Park Cut-Off Rd., CR 640, SR 17 |
| 325 | SR 208 at Osceola Co line | US 19 | 1937 | |
| 326 | SR 1 | Portland | 1937 | |
| 327 | SR 155 near Phillips Inlet | SR 115 | 1937 | |
| 328 | SR 40 in Walton Co | SR 33 | 1937 | |
| 329 | Pt. Washington | Eastern Lake | 1937 | |
| 330 | SR 198 at the Hillsboro Canal | SR 4 at Hillsboro Canal | 1937 | |
| 331 | Melbourne | Fellsmere | 1937 | |
| 332 | Fort Pierce | St. Lucie Co. line | 1937 | |
| 333 | White City | SR 8 | 1937 | |
| 334 | Williston | Lebanon | 1937 | |
| 335 | Brooker | SR 28 near New River | 1937 | |
| 336 | Tampa | Port Tampa | 1937 | |
| 337 | SR 13 N of Bronson | SR 19 E of Bronson | 1937 | |
| 338 | SR 47A SW of Palatka | SR 261 | 1937 | |
| 339 | East Palatka | St. Johns Co. line near Waldron | 1937 | |
| 340 | Orange Creek | SR 14 | 1937 |