| Route | From | To | Dates | Now |
| 221 | Sumner | Long Pond Schoolhouse | 1931 | |
| Designated "T. J. Yearty Highway" | ||||
| 222 | Stewart | SR 4 W of Port Sewall | 1931 | |
| 223 | Hobe Sound | Martin-Palm Beach Co. line | 1931 | |
| 224 | Jensen Beach | SR 4 and Old Dixie Highway | 1931 | |
| 225 | Ocala | Center Hill | 1931 | |
| 226 | Alabama state line S of Atmore, AL | Beulah Settlement | 1931 | |
| 227 | Perdido River near Barrineau Park | SR 190 at Coldwater Creek | 1931 | |
| 228 | Tampa Shores | Ozona | 1931 | |
| 229 | Safety Harbor | Dunedin | 1931 | |
| 230 | SR 64 W of Gandy Bridge | Point Pinellas | 1931-1945 | US 92, FL 687 |
| 231 | SR 15 in Pasco Co | SR 73 | 1931 | |
| 232 | Tampa | Hillsborough Co Line | 1931 | |
| 233 | SR 15 near Welch's Causeway | Long Key | 1931 | |
| 234 | SR 33 | Deerland | 1931 | |
| 235 | SR 46 S of Baker | Galliver | 1931 | |
| 236 | Ft. White | Mikesville | 1931 | |
| 237 | Belleview | Monroe's Corner | 1931 | |
| 238 | Santos | Orange Avenue | 1931 | |
| 239 | SR 2 near Ocala | Sumter Co line near Pedro | 1931 | |
| 240 | Zuber | Morriston | 1931 | |